Determination of Amino Acid Enantiomeric Ratios
by Gas Liquid Chromatography of the
N-Trifluoroacetyl-L-Prolyl-Peptide Methyl Esters

E. A. Hoopes, E. T. Peltzer and J. L. Bada
Amino Acid Dating Laboratory
Scripps Institution of Oceanography and Institute of Marine Resources
University of California, San Diego
La Jolla, CA 92093, USA.

Journal of Chromatographic Science (1978) 16: 556-560.

Received: 18 May 1978.
Revision received: 4 August 1978.
Published: November 1978

ABSTRACT

A technique for resolving amino acid enantiomers on a 12-foot packed column has been adapted to the analysis of fossils and other materials. The synthesis of the resolving agent, N-trifluoroacetyl-L-prolyl chloride (TPC), along with preparation of the solid support and other necessary reagents, is described. Representative chromatograms are presented, as well as data on the accuracy and precision of the technique.

Acknowledgements

This work was partially supported by grants from the National Science Foundation (EAR73-00320 and EAR77-14490) and the Office of Naval Research (N00014-75-C-0152). Jeffrey L. Bada is an Alfred P. Sloan Fellow 1975-1979.

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