Probe array development
Project Manager/Lead Scientist: Chris Scholin
This is the second year of a three-year project aimed at refining DNA probe array technology for use with the first- and second-generation Environmental Sample Processors (ESPs).
DNA probe arrays offer one means for detecting many molecular signatures simultaneously, thus providing a solution for rapid detection of multiple species and/or genes expressed in a single sample. Major emphasis during year one was directed towards refinement of probe attachment and signaling chemistries, given the sample processing capabilities and limitations of the first generation ESP. We accomplished stated milestones in 2003, emphasizing stabilization of reagents (for long term deployments), methods for automating array printing, and we have integrated suites of probes on several different “classes” of arrays that target harmful algal species, a number of benthic invertebrates (larvae), and bacterial and archaeal microbial groups. The design of probes for pelagic invertebrates is currently underway. Projected deployments of the probes in hand, as well as those currently in the design stage, include shallow water, stand-alone ESP deployments, as well as water-column and benthic MOOS/MARS experiments, as the latter platforms become available.