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1999 Projects
Current
Projects
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1999 Projects: Upper ocean biogeochemistry
Development and application of DNA probes
for detection of phytoplankton species
Project lead/manager: Chris Scholin
Project team: Roman Marin and
Peter Miller
The overall objective of this project is to develop a set of ribosomal RNA
(rRNA)-targeted DNA probes and methods for their application to speed and ease the
identification and quantification of certain eukaryotic phytoplankton. This work will
improve our understanding of organisms driving primary production in Central California
offshore waters and the manner in which those species respond to the ever-changing
physical and chemical environment. Work to date has proven that species-specific rRNA
probes are promising tools in this regard, particularly with respect to various toxic
phytoplankton linked to negative health effects on humans and wildlife worldwide. However,
a number of important research and development issues must be resolved if we are to
capitalize on these advancements to the greatest extent possible. First, to determine
whether probe strategies currently in use are applicable to a wider range of species than
the few targeted thus far, we must expand the repertoire of both species and
group-specific probes available. Second, we must conduct culture studies to determine the
extent to which change in the physiological status of cells alters their reactivity to the
probes. Third, to maximize the efficiency and utility of applying probes to large numbers
of samples, particularly when working aboard ship, we must improve the processes of data
collection, retrieval, and imaging. A three-year program will address these themes. Probes
developed will be evaluated in collaboration with Chavez et al. and MBARI’s upper
water-column research group.
Next: Molecular monitoring of picoplankton
Last updated: 07 October 2004 |
