Title
Monterey Bay Aquarium Research Institute
Marine Botany

Dinoflagellate Research Paper

by Josh Rapport

The following is a research paper written about the dinoflagellates. An index is provided for ease of perusal. Enjoy!

Index
I. The Paper

  1. History
  2. Morphology
    1. theca
    2. internal morphology
  3. Taxanomic Classification
    1. problems
    2. Ceratium
  4. Red Tides
  5. Toxins
    1. paralytic shellfish poisoning
    2. other toxins
    3. saxitoxin
    4. evolution of toxins
  6. Reproduction
  7. Economics
  8. Ecology
  9. Circadian Rhythms
  10. Bioluminescence
  11. Evolution

II. References
III. Acknowledgements

Dinoflagellates

History

In 1773 O. F. Muller became the first man to train the newly invented microscope on a dinoflagellate. Yet as early as 77 A.D. the Greek naturalist, Pliny, in a book of notes on his observations wrote, "There are sudden fires in the waters," most likely referring to what we now recognize as dinoflagellate bioluminescence (Nightingale, 1936). Dinoflagellates remained mysterious until F. Stein published his dinoflagellate monographs in 1878-1883, which provided the first good morphological description of the creatures. The brilliantly drawn figures show enough detail to allow modern taxonomists to classify the species Stein analyzed. To this day, many genera carry the name originally coined by Stein in his famous monographs. Since then, the list of identified dinoflagellates has exploded -- in just the last ten years the number of identified species has doubled -- and our understanding of the vital role they play has exploded with it. Still, much remains to be discovered.

Morphology

Morphologically, dinoflagellates consist of an epitheca and a hypotheca, separated by a transverse groove, the cingulum. Two flagella allow the single-celled, eukaryotic organism to propel itself through the water at speeds of up to 370mm/sec. (Spector, 1984). A longitudinal cleft called the sulcul groove, or sulcum, separates dorsal and ventral surfaces. The spiraling longitudinal flagellum inserts at the hypothecal end of the sulcum. The apex then denotes the opposite side. The second flagellum, the transverse flagellum, skirts the cingular groove like a ribbon.

Theca
Some species protect themselves with a plated covering called the theca, while others, called "naked" dinoflagellates, have a simple membranous cuticle. On the ultrastructural level the theca is composed of three membrane layers which can be either filled with cellulose or unfilled to lend different levels of stiffness to different species. The number of plates and their arrangement also varies across species and can be taxonomically useful. Unfortunately for the taxonomists morphologies can change drastically even within the same individual depending on environmental circumstances, degree of resolution, and life stage.

Internal morphology
Internally, dinoflagellates universally possess chlorophylls a and c (though some may possess different dimers of chlorophyll c with chlorophyll c2 predominating, and one report claimed that certain dinoflagellates may possess chlorophyll b and even phycobiliproteins). Like most algae, their adaption to the low intensity and filtered light that penetrates the sea comes from their accessory pigments -- mainly carotenes and xanthophylls, like peridinin and ß-carotene. They also possess various sterols (like cholesterol). Starch granules in the cytoplasm provide food storage along with long-chain fatty acids. They possess golgi bodies and vesicles, everything typifying a good eukaryote, except for the nucleus. A permanent nuclear envelope contains condensed, banded chromosomes, which is normal, but their DNA lacks histones and nucleosomes, giving them a "mesokaryotic" nucleus. Like the external morphology, the kinds and relative amounts of internal structures varies from species to species, and again may be different at different times even in the same individual.

Taxanomic Classification

Problems
Almost every taxanomically significant aspect in the dinoflagellates varies remarkably across species and genera, making identification highly labor and equipment intensive. New technologies are being developed to make the process easier. One new technique for cyst detection involves fluorescence tagging with primuline after treatment with methane(Yamaguchi, Itakura, Imai, and Ishida, 1995). Other taxonomic characteristics include lifestyle (parasitic vs. symbiotic vs. free living), life history, ultrastructure, and biochemistry. Even with highly sophisticated analyzing equipment, taxonomic classification can be a bear. This becomes especially problematic when trying to differentiate toxic or otherwise ecologically significant species from identical, yet less "important" counterparts. These problems, coupled with a poor fossil record, have made the story of how and when dinoflagellates evolved indecipherable. Speculations cover the full range of possibilities, and the story will remain a mystery until dinoflagellates appear in older fossil records, or until biochemical assays become more reliable.

Ceratium
In the Monterey Bay, dinoflagellate populations fluctuate seasonally. In a plankton tow taken from the Hopkins Marine Reserve (mesh size unknown) in mid-March, the majority of the dinoflagellates observed belonged to the phylum Ceratium, a thecate dinoflagellate with two long hypothecal horns extending apically, and one apical horn (see "morphology"). Most of the specimens observed in the tow may even belong to the same species. The homogeneity of the sample could have several causes. The species recovered measured about 175µm across and 575µm to the end of its long apical horn (unpublished data). Most species run from 10µm to 50µm, according to the literature, so a large mesh may have failed to trap the smaller species. However, very small diatoms, 20µm in diameter, appeared in the sample, suggesting that small mesh size was not a problem. Another cause may have been poor equipment, unable to resolve the smaller dinoflagellates. A third, and perhaps the most likely cause, requires a little background information.

Red Tides

Around the world, from polar to tropical seas, people have recorded a near-shore phenomenon known as a red tide. Darwin noted a red tide off the coast of South America in 1871 (Nightingale, 1936), and the number and range of reported red tides has increased over the last hundred years, simply due to an increasing awareness. The red colored cloud in the water near shore that characterizes a red tide comes from the aggregation of thousands of dinoflagellates. In one red tide in Olympia, Washington 15,834 individual cells jammed themselves into one milliliter of sea water (Nightingale, 1936). The number of different species of dinoflagellates in an aggregation during a red tide tends to be minimal, suggesting competition among different dinoflagellate species. Certain dinoflagellates secrete ectocrines, hormones that subdue other dinoflagellate species. Dinoflagellates often phagocytize other dinoflagellates. Many factors determine why one species dominates, and the relationships lack resolution as of yet, but the appearance of one predominant species in a net tow should not be too surprising.

Toxins

PSP
Worldwide attention was drawn to the red tide phenomenon when scientists linked the occurrence of red tides to another phenomenon known as paralytic shellfish poisoning (PSP), which has claimed human lives along with birds and other shellfish feeding animals. Since the discovery of toxic dinoflagellates, many different species with many different toxins have been characterized. To date, sixty toxin containing species have been described of the 2,000 extant species (Spector, 1984). Seven years ago taxonomists had described half as many toxic and non-toxic species.

Other toxins
Vast efforts have gone into this field since the discovery of PSP, yielding full characterization of about twenty toxins and their physiological effects. Besides PSP, cases of diarrhetic shellfish poisoning (DSP) and neurotoxic shellfish poisoning (NSP), as well as fish toxins have been ascribed to dinoflagellates. Toxins can be neurotoxic, hemolytic, or gastrointestinal in their physiological effect, and several are potentially lethal.

Saxitoxin
By far the most potent is the toxin implicated in paralytic shellfish poisoning. In California a rare episode of PSP killed several people and scared the California Department of Health Services (DHS) into placing an annual quarantine on shellfish as a protective measure. The toxin is called saxitoxin and acts by blocking sodium channels. This inhibits neuron depolarizations and action potentials, leading to respiratory failure. Thirteen derivatives of saxitoxin have been chemically resolved, and shellfish metabolism may cause interconversion between different forms of saxitoxin. This could convert a less potent toxin to lethal one. Shellfish also accumulate and concentrate the toxin, by filtering out the toxic dinoflagellates as they feed. PSP takes effect in as little as a half an hour depending on the species consumed and the concentration consumed (Spector, 1984). In Canada, in 1948, two four year old children ate toxic clams at the beach. They both died within minutes (Anderson, White, Baden). Despite intensive study, no anti-dote exists.

Toxin evolution
It is tempting to ask what the selection pressure might be for toxicity in a single-celled organism. Since even the biochemical pathway for toxin synthesis is unknown, it is difficult to speculate, but one interesting theory has been put forth. This theory suggests that the toxin may actually be used by the dinoflagellate for nitrogen storage. Chemically, saxitoxinoids are well suited for nitrogen storage, and the cyst form of most dinoflagellates seems to lack a nitrogen source (Spector, 1984). In this case, the toxicity of saxitoxin is incidental -- an unfortunate side effect. However, until more detail is uncovered about how the toxin is made and used by a dinoflagellate, the theory remains speculative.

Reproduction

Between blooms, the number of dinoflagellates found in the plankton can be exceedingly low. What prevents blooms from occurring? Why do blooms seem to occur according to a seasonal schedule? Grazing by copepods and nutrient limitation accounts for part of this, but blooms occur equally across nutrient and light gradients. The missing factor responsible for blooms is the cyst reservoir. The dinoflagellate cyst is an immotile stage in their life history. Dinoflagellates spend the majority of their life in a haploid, vegetative state, reproducing asexually by cellular mitosis -- one dinoflagellate pinches off to form two. (Thecate species have to deal with re-formation of the theca, and different species use different methods, which taxonomists can use for classification.) Given an environmental cue, two of the newly formed dinoflagellates may fuse as gametes to form a "planozygote," which retains both nuclei and both longitudinal flagella. Various laboratories have investigated the environmental cues, and while low nitrogen is used almost universally in vitro for beginning the sexual cycle, one report indicated that all of the following may induce fusion under laboratory conditions: low nitrogen, low phosphorous, low light intensity, shortened day length, decreased water temperature, and increased salinity (Faust, 1992). Most likely a combination of these act as the environmental cues in vivo, and all of these are symptomatic of winter in the temperate seas. The planozygote continues the cycle with a series of morphological changes to become the cyst, or hypnozygote. It loses all flagella and grows in size, often thickening its theca by depositing cellulose between thecal membrane layers. Metabolically active cytoplasm is reduced and replaced with starch granules. In the nucleus, protective proteins congregate around the chromosomes (Xiaoping, Dodge, and Lewis, 1989). Presumably these changes prepare the dinoflagellate to overwinter by slowing metabolism, augmenting food storage, and protecting against agitation. When they lose their motility, they sink, and it has been shown in vitro that a resting cyst under ideal conditions may remain viable for five-and-a-half years (Anderson, White, and Baden, 1985). Cysts build up in the sediment over the years to form a "cyst reservoir" from which new dinoflagellates are recruited to initiate a bloom. A temperature rise induces ecdysis, or excystment, and the hypnozygote reverts to the vegetative state, reforming both flagella. Where in the cycle meiosis occurs appears unclear. Indeed it may vary from species to species, and imaging of the resting cyst has proven difficult due to the thickened theca. But when the excysted cell emerges it is haploid, like the initial vegetative cell. Putting everything together, the vegetative cells gradually sink out of the plankton with the onset of fall, (or generally poor living conditions) leaving a limited number of free-floating individuals in the plankton. Grazing rates or nutrient limitation keep the population from exploding at this point. Then as spring sets in and temperatures increase, hypnozygotes excyst and new dinoflagellates are recruited. The cyst reservoir contains a huge number of hypnozygotes -- residuals from not just the previous winter, but from as many as five seasons past. Given the right conditions -- high nutrient level, high light level, low grazing, and low competition from other bloom species -- cysts ecdyze by the thousands. With a recorded growth rate of as much as one division per day, it only takes 12.5 cysts per square centimeter to initiate a bloom (Anderson, White, and Baden, 1985). In the Monterey Bay, spring ocean conditions are markedly turbid. Due to a relatively high nutrient level left over from turbulent mixing by the winter storms coupled with an increasing light intensity with the onset of summer, this turbidity is a result of increased phytoplankton growth. These conditions often lead to visible red tides. In the spring of 1995 a Gymnodinium species developed into a red tide in the Monterey Bay (personal correspondence with Deborah Robertson). This happens to be a non-toxic species, so no special quarantines were placed on shellfish this season (personal correspondence with the California DHS).

Economics

When the bloom species is toxic, mussels, scallops, clams and other filter-feeding bivalves all may be affected. DHS of California places a quarantine on potentially toxic shellfish from May 1 through October 31, in addition to constant monitoring. Worldwide monitoring programs are in effect, and in parts of Alaska year-round bans of shellfish have been in effect since 1947. In Japan, red tides devastate the pearl industry. Fish kills due to ciguatoxin -- a dinoflagellate toxin that infects fish directly -- induce low yields, while bad publicity due to PSP can affect fishing industries, even if the toxin has no direct effect on the fish.

Ecology

Ecologically, of course, dinoflagellates and blooms incur large effects as well. As primary producers dinoflagellates must contribute to world CO2 consumption significantly, though not nearly as much as their numerous counterparts, the diatoms. Diatoms grow much faster than dinoflagellates, however they sink much faster. Hence, in turbid areas such as the temperate and polar regions, where stirring holds the diatoms in the upper water column, they dominate significantly over dinoflagellates. In the tropical seas, where turbulence is at a minimum, dinoflagellates predominate in the plankton. The slower growth rate of dinoflagellates, as well as a limited nutrient base, contributes to the stereotypical clarity of tropical seas. Because dinoflagellates use their flagella for motility they can vertically migrate -- up during the day when the light is strongest, and down at night -- which has consequences for the copepods who feed on them and on up the food chain.

Circadian Rhythms

Daily migration patterns have also been given significant attention because of their association with circadian rhythms. In order for a behavior to qualify as controlled by a circadian rhythm it must be shown in vitro to occur without environmental cues. For example, dinoflagellates have been shown to modify photosynthetic rate during the course of a day. A dinoflagellate kept in sunlight for 24 hours will fix the most carbon towards the afternoon and will fix much less carbon at "night," even though the light intensity has not changed (Spector, 1984).

Bioluminescence

Because of their fast reproductive rate and their bioluminescence, dinoflagellates make excellent subjects for circadian rhythm studies. At night glowing blue flecks bounce off divers, and sailors have reported seeing the iridescent outline of otherwise dark boats. The bioluminescence is produced by different, fairly well studied biochemical reactions that produce visible light. The chemicals involved in the reaction vary across species. Apparently, bioluminescence has remarkably evolved many times, independently. These chemicals make excellent biological tags for other biochemical experiments, making bioluminescent dinoflagellates useful as model systems. Again it is tempting to ask why something like bioluminescence may have evolved, and why so many times. Today one can only speculate, for the true selection pressure remains elusive.

Evolution

Perhaps the answer to evolutionary questions lies in certifying the phylogenetic classification. Without a fossil record, the inferences made are strained and derived, and unprovable. Suggestions have been made that dinoflagellates may bridge the gap between prokaryotes and eukaryotes, may be much older than the current fossil record reveals, may have evolved into brown algae, or may represent their own unique evolutionary line. Other maybe's abound, and now, two-hundred years since their discovery, much about the dinoflagellates remains mysterious.

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Copyright Josh Rapport 1996

Last updated: Feb. 05, 2009