Traditionally, identification of species within Pseudo-nitzschia has been accomplished through scanning electron microscopy. This is, however, an arduous and time-consuming process that involves an expert taxonomist. More efficient identification methods have been adopted, especially in light of the necessity to prevent Amnesic Shellfish Poisoning (see Toxin page) outbreaks. Two methods have been developed that expedite this process: the Whole Cell Hybridization Method and the Sandwich Hybridization Method. Both methods target ribosomal RNA (rRNA) since each species of Pseudo-nitzschia contains unique sequences.
Whole Cell Hybridization Method
Sandwich Hybridization Method
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copyright Jennifer Shin 1999.