Monterey Bay Aquarium Research Institute

Helena Solo-Gabriele, Ph.D.
Associate Dean for Research
Civil, Architectural, and Environmental Engineering
University of Miami

Simultaneous concentration of bacteria, protozoans,
and viruses in marine waters using a dual layer filtration system

Wednesday – August 1, 2007
Pacific Forum – 3:00 p.m.

The ability to rapidly and effectively concentrate diverse microbes is an essential component for monitoring water quality at recreational beaches. The purpose of this study was to develop a 90 mm diameter 0.45µm pore size dual membrane system, which can simultaneously concentrate both viruses and either bacteria and/or protozoa. The top PVDF membrane was used to filter bacteria/protozoa by physical straining while the bottom HA membrane retained viruses through adsorption. Results indicated that 73+50% of the test bacteria (E. faecalis) and 82+8% of the test virus (MS2 coliphage) were recovered. Recoveries of Cryptosporidium and Giardia varied between 40 and 70%. The highest recoveries of poliovirus (Polio 1 CHAT) were obtained with a guanidinium hydrochloride elution solution followed by secondary concentration using a commercially available column. Future experiments should focus on measurements utilizing additional bacteria and viruses as well as water with different physical and chemical parameters.


Next: August 23 - 11th Annual Intern Sympoium.