Monterey Bay Aquarium Research Institute


Life-on-a-chip for multi-parameter analysis

of microbial populations

Deirdre Meldrum, Ph.D., University of Washington

Friday - June 16, 2006

12:00 pm - Pacific Forum

Ecogenomic sensors take advantage of genome and proteome technologies developed in the Human Genome Project to discover who is in the ocean (e.g. what microbes live there), what are they doing, and how do they respond to events in the oceanic environment – such as earthquakes, volcanoes, toxic algae blooms (phytoplankton), as well as changes in the carbon, pH, and temperature. We are leveraging technology from the Microscale Life Sciences Center (MLSC) to create ecogenomic sensors. The MLSC is developing microscale instrument modules to measure multiple parameters in single living cells in real time to correlate cellular events with genomic information. Current capabilities in live cells include measurement of substrate-dependent O 2 consumption rates and measurement of expression from multiple genes using fluorescent reporters, while in fixed cells we are developing the ability to carry out qPCR and qRT-PCR on multiple genes simultaneously and to generate single cell proteomics profiles.