Development of microbiochemical systems and their application
to deep sea in-situ genetic analysis

Teruo Fujii, Ph.D.
University of Tokyo

Friday, October 19, 2001
12:00 Noon–Pacific Forum







Capillary electrophoresis chip with integrated electrodes

In order to realize total reaction and analysis systems, we have been developing PDMS (polydimethylsiloxane) based microbiochemical systems. Several components, such as microreactors, a small volume liquid handling chip, and capillary gel electrophoresis chips, are fabricated through molding processes against negative masters fabricated with SU-8 photoresist on silicon wafers. This year we have concentrated on the integration of the temperature control devices on a glass substrate that forms a hybrid structure bonded with the PDMS microchip. The integrated chip could be used for continuous-flow PCR, which is one of the indispensable reactions for the in-situ gene analysis. The detailed designs, fabrication processes, and chemistries will be discussed in the presentation.

Next: The Comparative Genomics Program at the Joint Genome Institute