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Development of microbiochemical systems and their application to in situ genetic analysis systems for deep-sea microorganisms

Teruo Fujii, Ph.D.
University of Tokyo

Wednesday, September 13, 2000
3:00 p.m.—Pacific Forum

Development of Microchips for Capillary Electrophoresis

In order to realize total reaction and analysis systems, we have been developing a PDMS (polydimethylsiloxane) based microbiochemical system. Several components, such as microreactors, a small volume liquid handling chip and capillary gel electrophoresis chips are fabricated through molding processes against negative masters fabricated with SU-8 photoresistors on silicon wafers. Based on the knowledge and techniques acquired through these developments, this year we have launched a new project to develop in situ genetic analysis systems for deep-sea biological exploration. The system prototype consists of a sampling and extraction part, a gene amplification part, a separation part with integrated detectors, and a fraction collection and functional characterization part.

The detailed designs, fabrication processes, and chemistries will be discussed in the presentation.

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