Microfluidic enzyme assays and sub-microliter sample preparation for DNA sequencing

Andrew Hadd, Ph.D.
Molecular Dynamics
Palo Alto, California

Wednesday, November 10, 1999
3:00 p.m.—Pacific Forum

Slide2.GIF (7751 bytes)Microchips have been recently developed for performing a wide-range of analytical measurements and represent the ability to miniaturize current "bench-top" experiments with increased speed, automation, and volumetric reduction of sample and waste. Within the channel network of a glass microchip, cross intersections and mixing tees are used for valving and dispensing picoliter volumes with high volumetric reproducibility (0.3% RSD). I will describe the development of an automated, microchip assay for -galactosidase using a fluorogenic substrate. Another critical limitation of microfabricated devices is the interface of macro-prepared samples with the microchip environment. Towards improving the interface and compatibility of samples with microchip analysis, a sub-microliter DNA sequencing device has been developed for both capillary array electrophoresis and microchip analysis. To meet the cost-reduction demands of sample preparation for both capillary array electrophoresis and microchip-based instruments, a 16-channel system was developed for 500 nL dye-primer and dye-terminator DNA sequencing reactions. The analysis of sub-microliter DNA sequencing samples by this 16-channel microchip device will also be presented.

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 Last updated: December 19, 2000