5 June 2001
New instrument enables remote detection
of toxic algae in real time
MOSS LANDING, California— Using the Environmental Sample
Processor (ESP), the first in a new class of oceanographic research
instrumentation, a team of MBARI scientists and engineers detected the
onset and development of a harmful algal bloom (HAB). The ESP was deployed
in May as part of an ongoing, multi-institution research effort in the Gulf of
Maine aimed at understanding the growth, transport, and decay of toxic
blooms which can adversely affect the health of humans and wildlife. The
toxic dinoflagellate, Alexandrium fundyense, was detected during
the experiment at low densities of approximately 100 cells per liter of
seawater.
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ESP DNA probe array revealing the presence of two
different species
of toxic algae. The two large bright spots near the top and
left of the image are orientation registration marks. The four large
spots near the bottom of the image are probes specific for Alexandrium
tamarense,
a dinoflagellate associated with the human disorder known as
paralytic shellfish poisoning. The four faint spots near the top of
the image are probes specific for Pseudo-nitzschia australis,
a diatom
known to produce the neurotoxin domoic acid. |
The ESP was developed at the Monterey Bay Aquarium Research Institute (MBARI)
and is designed to autonomously collect discrete subsurface water samples
and concentrate microorganisms contained within those samples onto filter
disks. The ESP automates application of preservatives as well as DNA and
other molecular probes to the collected samples to enable identification
and quantification of particular species captured. In addition to
archiving discrete samples for microscopy and nucleic acid and toxin
analyses, the instrument also transmits results of probe assays in
real-time to a remote location for data processing and interpretation.
This capability provides an entirely new means of remotely detecting
microorganisms in the environment, enabling water quality assessments in
the absence of ship-based sample collection and time-consuming laboratory
analysis.
The ESP is the result of four years of teamwork between MBARI
scientists and engineers, under the leadership of Chris Scholin and Gene
Massion. Although the current instrument is targeted at HAB research and
issues related to the health of humans and marine wildlife, it is capable
of emulating a wide variety of water quality measurements.
"The exciting results from the Gulf of Maine experiment are the
culmination of four years of effort at MBARI in developing the capability
for in situ detection of microrganisms in the marine environment
using genetic probes," said Marcia McNutt, MBARI president and CEO.
"The fantastic teamwork between MBARI scientists and engineers was
instrumental in harnessing a new technology to address a fundamental
problem in marine research that also has important implications for human
health. We are optimistic that the ESP will usher in a new era in our
ability to understand the ecology of the microscopic life in the
oceans."
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Media Contact:
Debbie Meyer, ( 831) 775-1807, pressroom@mbari.org
Images
related to this news release.