Point Lobos Protocols

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Filtered Samples

1. Chlorophyll : 11 depths, 0m 5m 10m 20m 30m 40m 60m 80m 100m 150m 200m
2. Chlorophyll 1m Fractionations : 1 depth, 0m
3. Chlorophyll 5m Fractionations: 1 depth, 0m
4. T0 0.1%: 1 depth, Light Penetration Depth = 0.1% as determined by Secchi Index
5. T0 100%: 1 depth, 0m (Note T0=Time Zero, treated with C14 and processed ASAP)
6. C14 at light penetration depths (re:Secchi), 100%,50%,30%,15%,5%,1%, and 0.1%. Water samples are incubated first then filtered. Store bottles in corresponding light level incubator sleeves.
7. POM: 1 depth, 0m from Underway Mapping System (POM=Particulate Organic Matter). Note temperature, GMT, Volume Filtered on NASA Data Sheet in Cruise Notebook.
8. POC: 1 depth, 0m. POC=Particulate Organic Carbon. Use combusted GFF filter, fold filter and store in Glassine envelope, record volume filtered on envelope.
9. A*: 4 depths, 0m 10m 20m 40m. Store in cryovial, then in Nitrogen . At Mooring1 only.

Unfiltered Samples

1. Nutrients : At Mooring1: 11 depths, 0m 5m 10m 20m 30m 40m 60m 80m 100m 150m 200m. At C1 and Mooring2: 0m only.  Store vials in freezer. 
2. Phytoplankton: 0m sample in plastic duct taped bottles.
3. FCM: 0m scint. vials. Store in toxic cooler.
4. Salinity: 0m sample in glass bottles. 1 from CTD, 1 from underway flow.  Store in cooler.
5. DIC (Dissolved Inorganic Carbon) - 0m sample in dark brown bottle with yellow tape.   NASA sample.  Store in cooler.

Note: Any deviation from the above procedures should be noted on the Data Sheet. i.e. Volume Fitered is more/less than required, or incubation is 6 hours, no c14 sample taken, etc.