Point Lobos Quick Reference Chart for Shipboard Sample Collection

Filtered Samples

	Sample	Filter	Vol	Container	Bath	Amt	Storage	Notes
1	Chl	GFF	280ml	scint.vials	acetone	10mls	freezer	Keep in dark
2	Chl frac. 1m	1m nuclepore	100ml	scint.vials	acetone	10msl	freezer	Keep in dark
3	Chl frac. 5m	5m nuclepore	100ml	scint.vials	acetone	10msl	freezer	Keep in dark
4	T0 0.1%	GFF	280ml	scint.vials	none	n/a	freezer	Treat w/C14
5	T0 100%	GFF	280ml	scint.vials	none	n/a	freezer	Treat w/C14
6	POM	CombustedGFF	4L	petri dish	none	n/a	freezer	Use the big filters
7	POC	CombustedGFF	500ml	glassine	none	n/a	freezer
8	A*	GFF	1L	cryovial	none	n/a	nitrogen	Nitrogen in Thermos

1. Chlorophyll : 11 depths, 0m 5m 10m 20m 30m 40m 60m 80m 100m 150m 200m

2. Chlorophyll 1m Fractionations : 1 depth, 0m

3. Chlorophyll 5m Fractionations: 1 depth, 0m

4. T0 0.1%: 1 depth, Light Penetration Depth = 0.1% as determined by Secchi Index

5. T0 100%: 1 depth, 0m (Note T0=Time Zero, treated with C14 and processed ASAP)

6. C14 at light penetration depths (re:Secchi), 100%,50%,30%,15%,5%,1%, and 0.1%. Water samples are incubated first then filtered. Store bottles in corresponding light level incubator sleeves.

7. POM: 1 depth, 0m from Underway Mapping System (POM=Particulate Organic Matter). Note temperature, GMT, Volume Filtered on NASA Data Sheet in Cruise Notebook.

8. POC: 1 depth, 0m. POC=Particulate Organic Carbon. Use combusted GFF filter, fold filter and store in Glassine envelope, record volume filtered on envelope.

9. A*: 4 depths, 0m 10m 20m 40m. Store in cryovial, then in Nitrogen . At Mooring1 only.

10. Nutrients : 11 depths, 0m 5m 10m 20m 30m 40m 60m 80m 100m 150m 200m at Mooring1 only, 0m depths at C1 and Mooring2. Nutrients are not filtered. Store vials in freezer.

Note: Any deviation from the above procedures should be noted on the Data Sheet. i.e. Volume Filtered is more/less than required, or filter dropped on table, etc.