Point Lobos Quick Reference Chart for Shipboard Sample Collection

Filtered Samples

 

 

Sample

Filter

Vol

Container

Bath

Amt

Storage

Notes

 

1

Chl

GFF

280ml

scint.vials

acetone

10mls

freezer

Keep in dark

 

2

Chl frac. 1m

1m nuclepore

100ml

scint.vials

acetone

10msl

freezer

Keep in dark

 

3

Chl frac. 5m

5m nuclepore

100ml

scint.vials

acetone

10msl

freezer

Keep in dark

 

4

T0 0.1%

GFF

280ml

scint.vials

none

n/a

freezer

Treat w/C14

 

5

T0 100%

GFF

280ml

scint.vials

none

n/a

freezer

Treat w/C14

 

6

POM

CombustedGFF

4L

petri dish

none

n/a

freezer

Use the big filters

 

7

POC

CombustedGFF

500ml

glassine

none

n/a

freezer

 

8

A*

GFF

 

1L

cryovial

none

n/a

nitrogen

Nitrogen in Thermos

 

1.      Chlorophyll : 11 depths, 0m 5m 10m 20m 30m 40m 60m 80m 100m 150m 200m

2.      Chlorophyll 1m Fractionations : 1 depth, 0m

3.      Chlorophyll 5m Fractionations: 1 depth, 0m

4.      T0 0.1%: 1 depth, Light Penetration Depth = 0.1% as determined by Secchi Index

5.      T0 100%: 1 depth, 0m  (Note T0=Time Zero, treated with C14 and processed ASAP)

6.      C14 at light penetration depths (re:Secchi), 100%,50%,30%,15%,5%,1%, and 0.1%.  Water samples are incubated first then filtered.  Store bottles in corresponding light level incubator sleeves.

7.      POM: 1 depth, 0m from Underway Mapping System (POM=Particulate Organic Matter).  Note temperature, GMT, Volume Filtered on NASA Data Sheet in Cruise Notebook.

8.      POC: 1 depth, 0m.  POC=Particulate Organic Carbon. Use combusted GFF filter, fold filter and store in Glassine envelope,  record volume filtered on envelope.

9.      A*: 4 depths, 0m 10m 20m 40m.  Store in cryovial, then in Nitrogen .  At Mooring1 only.

10.  Nutrients : 11 depths, 0m 5m 10m 20m 30m 40m 60m 80m 100m 150m 200m at Mooring1 only, 0m depths at C1 and Mooring2.  Nutrients are not filtered.  Store vials in freezer.

 

Note: Any deviation from the above procedures should be noted on the Data Sheet.  i.e. Volume Filtered is more/less than required, or filter dropped on table, etc.