Detection of Pseudo-nitzschia australis

1

1a

4

4a

Figure 2. Examples of the detection of the toxic, pennate diatom Pseudo-nitzschia australis with the shipboard ESP during MUSE. The presence of the diatom results in the emission of light. P. australis was not detected in sample 1, which was collected in the area of upwelling, but was detected in sample 4 (middle left), where the water was warmer. Samples were collected for later whole cell analyses. Panel 1a was treated to show all species in the sample, whereas sample 4a was treated with a probe that labels P. australis specifically. These analyses confirmed the predictions made based on the real-time data collected on the ship. Map shows where samples 1 and 4 were collected in relation to ocean temperature.