The Environmental Sample Processor (ESP) was deployed in Monterey Bay on October 3, 2002, 24 hours after this survey and recovered October 10.
The position and depth of the ESP is shown below as the blue dot under the yellow triangle. This provides some context for characterizing the environment into which the ESP was deployed. Chlorophyll was swept out of the bay September 27-30 by a low-salinity mesoscale filament that originated from the north. This is a very interesting story - stay tuned for more on that later from the Ryan lab. Chlorophyll remained relatively low through early October.
For our deployment in Monterey Bay, 2002, we used two different types of DNA probe arrays.The HAB (harmful algae bloom) arrays had probes for several different species of harmful and toxic phytoplankton. We use these to look for multiple harmful species simultaneously in the same sample. The SSU (small subunit rNA) arrays had probes that target universally conserved sequences (all organisms), all barnacles (general, group-specific), a specific species of barnacle, and pennate diatoms (general, group -specific). The arrays shown below illustrate tests to assess specificity of a universal probe (U519), the generic barnacle probe (B1066) and the barnacle species-specific probe (B915). When one of these arrays is treated with extracts from the particular species of barnacle targeted, all the spots light up (B915, B1066, U519). When one of these arrays is treated with extracts from a barnacle other than the specific species targeted, only the B1066 and U519 spots light up as expected.