Monterey Bay Aquarium Research Institute
2nd Generation Environmental Sample Processor (ESP)
Field Experiments
HAB 2006
In situ detection of Pseudo-nitzschia spp. and domoic acid (DA) in Monterey Bay March 2006 using the ESP.  A) DNA array with probes for Pseudo-nitzschia australis, P. multiseries / pseudodelicatisima, Alexandrium catenella, and Heterosigma akashiwo (HAB array). Only control spots (black rectangles) are visible.  Targeted species are below detection level.  B) Protein array for DA, corresponds to (A).  Blue ringed spots are IgG controls confirming consistency of detection chemistry.  Spots in red rectangles are DA conjugate.  Maximum intensity in DA conjugate spots indicates DA levels are below detection limit, whereas decreasing spot intensity indicates presence of DA (competitive ELISA).  C) HAB array from a later time indicates the presence of P. australis (yellow bounded spots) and P. multiseries / psuedodelicatissima (green bounded spots), both of which can produce DA.  D) DA array that corresponds to (C); the DA conjugate spots are considerably dimmed, indicating the presence of this toxin.  From Greenfield et al. Limnology and Oceanography: Methods (in review), and Doucette et al. (in prep)

Last updated: Feb. 04, 2009