Monterey Bay Aquarium Research Institute
Environmental Sample Processor (ESP)
Second Generation (2G) ESP pucks and carousel

Pucks

A puck may hold a probe array (for analytical purposes) or serve some other function. There are several types for different purposes, but all have the same external shape and size for handling by ESP mechanics.

Analysis of samples takes place in the pucks. When the puck is held in the clamp, water samples and reagents are pumped through in programmed sequences to perform an analysis (such as SHA or ELISA).

The styles of pucks currently available are shown below.

puck: low sample volume

Low sample volume

puck: high sample volume

High sample volume

puck: sample archival

Sample archival

puck: large format array

Large format array

puck: small format array

Small format array

 

Some of the functions of pucks are:

  • Pop-top – for imaging a DNA array
  • Wet-storage – built in valve allows fluid to be stored
  • Dry-storage – to store samples which may be dried
  • Filter – to clean lysis prior to moving fluid to processing
  • Custom - for future processes

Carousel

The carousel is a circular array of seven tubes which hold the pucks. For a deployment, six of the tubes are filled with pucks, and one is left empty. As the instrument goes through a sequence of operations and analyses, used pucks are dropped into the empty tube. As that tube fills, another is emptied, and so on.

carousel drawing carousel photo
3-D drawing showing the carousel
Photo of the carousel

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2G ESP
Probe Arrays

ESP Technology

Last updated: Feb. 04, 2009